Sunday, December 16, 2018



A ‘Self-Aware’ Fish Raises Doubts About a Cognitive Test
A report that a fish can pass the “mirror test” for self-awareness reignites debates about how to define and measure that elusive quality.
[[My sarcastic comments in double brackets.]]

Very few animals have ever passed the mirror test for self-recognition — even most primates fail it. The news that a fish seemed to recognize itself in one recent study has made psychologists and animal behaviorists wonder anew what (if anything) the mirror test proves.



December 12, 2018


A little blue-and-black fish swims up to a mirror. It maneuvers its body vertically to reflect its belly, along with a brown mark that researchers have placed on its throat. The fish then pivots and dives to strike its throat against the sandy bottom of its tank with a glancing blow. Then it returns to the mirror. Depending on which scientists you ask, this moment represents either a revolution or a red herring.
Alex Jordan, an evolutionary biologist at the Max Planck Institute for Ornithology in Germany, thinks this fish — a cleaner wrasse — has just passed a classic test of self-recognition. Scientists have long thought that being able to recognize oneself in a mirror reveals some sort of self-awareness, and perhaps an awareness of others’ perspectives, too. For almost 50 years, they have been using mirrors to test animals for that capacity. After letting an animal get familiar with a mirror, they put a mark someplace on the animal’s body that it can see only in its reflection. If the animal looks in the mirror and then touches or examines the mark on its body, it passes the test.
Humans don’t usually reach this milestone until we’re toddlers. Very few other species ever pass the test; those that do are mostly or entirely big-brained mammals such as chimpanzees. And yet as reported in a study that appeared on bioRxiv.org earlier this year and that is due for imminent publication in PLOS Biology, Jordan and his co-authors observed this seemingly self-aware behavior in a tiny fish.
Jordan’s findings have consequently inspired strong feelings in the field. “There are researchers who, it seems, do not want fish to be included in this secret club,” he said. “Because then that means that the [primates] are not so special anymore.”
If a fish passes the mirror test, Jordan said, “either you have to accept that the fish is self-aware, or you have to accept that maybe this test is not testing for that.”
[Yes, yes yes!! I have been saying this for years. Self-awareness indeed! Because it can find a spot on its own body via the mirror image.

The correct explanation may be a little of both. Some animals’ mental skills may be more impressive than we imagined, while the mirror test may say less than we thought. Moving forward in our understanding of animal minds might mean shattering old ideas about the mirror test and designing new experiments that take into account each species’ unique perspective on the world.
Reflecting on Primates
The evolutionary psychologist Gordon Gallup thought up his field-defining experiment while shaving in a mirror one day as a graduate student. When Gallup took a position at Tulane University a little later, he had access to animals at the Delta Regional Primate Research Centerhe could test his idea on.
Gallup started by showing a mirror to four chimpanzees, each alone in a cage. At first the chimps reacted as if they were seeing a stranger. But after a few days, they stopped threatening and vocalizing at the reflections. They started using the mirrors to look at themselves: They cleaned food from their teeth, picked their noses and examined their genitals. To prove that the chimps understood what they were seeing, researchers anesthetized the animals and dabbed red dye onto their eyebrows and ears. Then they returned the chimps to the mirrors. Looking at their reflections, the animals touched their fingers to the paint on their faces.
What surprised Gallup more than the chimpanzees’ success at recognizing themselves was the failure of macaques he tested at the same time. When the paper came out in Science in 1970, “it was bigger than I thought it would be,” Gallup said. “People were quite taken with the finding.”

Gordon Gallup, an evolutionary biologist now at the State University of New York, Albany, invented the mirror test for self-recognition almost 50 years ago. To him, the only animals that have definitively passed it are humans, chimpanzees and orangutans.
Courtesy of Gordon Gallup
We were speaking in his cramped office on the campus of the State University of New York, Albany, where Gallup has worked since 1975. Every surface and drawer overflowed with stacks of paper. A phone teetered atop a paper heap that covered the entire desk. Here and there, obsolete technologies peeked through the clutter: a dusty vintage computer scattered with floppy disks, VHS tapes on a rolling TV cart, a slide projector. Gallup sat on a rolling desk chair that had worn a circular hole through the carpet to the industrial floor below.
He showed me black-and-white photos of chimps studying themselves in mirrors. What the mirror test shows, Gallup said, is self-awareness, which he defines as “the ability to become the object of your own attention.”
[[Wow. “The object of your own attention.” But what is the “you” here? A person?  Who says so?]]
 And he believes this implies a certain rare intellect. Any animal that can recognize itself in a mirror, Gallup thinks, can potentially recognize that others have their own minds and even empathize with them.
[[Wow. Recognize that others have minds! Empathize!! Who says so? This is all overblown ffantasy.]]
A sense of self means a sense of selves.
Around the same time as Gallup’s initial study, the psychologist Beulah Amsterdam, at the University of North Carolina, Chapel Hill, was working on a similar experiment with babies and toddlers, in which she dotted their noses with rouge. She found that most children recognize themselves in a mirror by age two. In the following years, Gallup and his colleagues tested a range of other animals with mirrors, from primates to chickens, and found more failures than successes. Most animals never moved beyond seeing the reflection as another animal.
But a few did — or seemed to. Diana Reiss, a marine mammal scientist and cognitive psychologist at Hunter College in New York City, has done extensive research on dolphins, including mirror tests both with Gallup and other co-authors. Though the study she worked on with Gallup wasn’t conclusive, she said, later studies showed that dolphins can pass the test. In their reflections, aquarium dolphins studied their eyes and mouths, did flips and blew different kinds of bubbles. After being drawn on with black marker, the dolphins spent more time looking at the marked sides of their bodies in the mirror.


As observed through one-way glass, a dolphin playfully spins in front of its reflection. Some researchers consider dolphins to have passed the mirror test, but that conclusion is debated.
Courtesy of Diana Reiss
Monkeys, for the most part, have continued to fail mirror tests. Some rhesus macaques passed after weeks of training with their heads restrained, forced to stare at the mirror. In another experiment, researchers tried marking marmosets with chocolate to increase their motivation, with no luck. (Some of the monkeys tried to lick the chocolate in the mirror.) But Reiss and her colleagues have found mirror self-recognition in Asian elephants. Orangutans, bonobos and gorillas have all passed the test, too, Reiss said — along with one bird, the magpie.
In Gallup’s view, though, only three species have definitively passed: chimpanzees, orangutans and humans. He finds the evidence for every other species uncompelling, and thinks researchers are reading things into animals’ behavior that aren’t there. Gallup has co-authored papers critiquing others’ methods and interpretations.
One researcher whose results Gallup challenged was the Harvard University biologist Marc Hauser, who charmingly marked monkeys called cotton-top tamarins by dyeing their fluffy white hair exotic colors. Hauser and his co-authors reported that the monkeys touched their heads while looking in the mirror. Yet an attempted replication of the study failed, and in 2011 Hauser left Harvard after an investigation found he had falsified data in other studies.
Still, Gallup claimed he keeps an open mind. “I’m more than happy to consider the possibility that any other species might be capable of recognizing itself in a mirror,” he said.
Enter Jordan’s fish.
Social Enough to Be Self-Aware
Jordan is interested in the mental skills that animals lose or gain as they evolve to live in social groups. He and his co-authors wanted to explore the cognitive limits of social fish — so they thought of the mirror test. First they tested cichlids, which didn’t pass. So the researchers pondered what fish to try next. “The answer came: Of course it should be the cleaner wrasse,” Jordan said. “It is an incredibly intelligent animal, and highly social.”
Cleaner wrasse live on coral reefs and specialize in nibbling parasites and dead skin off the bodies of larger fish that could easily make a meal of them. It’s a dangerous life, and the wrasse have to be savvy to avoid being eaten themselves. In the lab and in the wild, Jordan said, the fish are inquisitive about their environments and attentive to humans, attempting to clean a person’s hands or face masks as they would a client.
In front of a mirror, cleaner wrasse seemed to pass through the same stages as chimpanzees. First they attacked their reflections. Then they performed unusual behaviors in front of the mirrors, like swimming upside down. After several days, the fish were spending extra time near the mirrors, as if studying their reflections.

Alex Jordan, an evolutionary biologist who studies behavior at the Max Planck Institute for Ornithology in Germany, was inspired to test the cognitive limits of social fish, so he administered a mirror test to the fish called a cleaner wrasse.
Simon Gingins
Next, the researchers marked the fish that seemed to be catching on. They injected a bit of brown material (or clear, for a control) under the skin of each fish’s throat. Afterward, some of the fish seemed to study the marks in front of the mirror. Then they scraped their throats against rocks or the sandy bottom of their tanks — a common fish behavior for removing irritants, Jordan said. The fish often followed this maneuver by swimming back up to the mirror. Three out of the four fish that made it this far in the study passed the mirror test, the authors concluded.
The researchers spent more than three years trying to get the paper published. Peer review is a largely cloaked process in which experts in a field respond anonymously to papers that have been submitted to journals. But Gallup signed his reviews of the cleaner wrasse paper, which were “violently anti,” Jordan said.
In Albany, Gallup chuckled at the suggestion that the fish had recognized themselves. To him, the demonstrated behavior was too ambiguous. He wrote in one of his reviews that when a wrasse scraped its throat, maybe it was pantomiming an instruction for what the mirrored fish should do — as in “You’ve got some mustard on your chin,” said Jordan, who called this alternate explanation “incredibly far-fetched.”
I think the community wants a revision and a reevaluation of how we understand what animals know.
Alex Jordan, Max Planck Institute for Ornithology
Reiss also reviewed the paper several times for different publications, she said. She wasn’t convinced that behaviors like swimming upside down showed that fish were testing how the mirror worked. She and Gallup also found it problematic that the brown mark resembled a parasite — to which wrasses instinctively react — unlike the unnatural marks on other animals. “I think for a claim like this, the evidence has to be much stronger,” Reiss said.
In response to the reviewers’ objections, Jordan and his co-authors added more control experiments to their study. Now that the paper has finally been accepted for publication, Jordan thinks the grueling revision period made the study stronger. “And, you know, I didn’t die in the process,” he joked.
Alexandra Horowitz, a psychologist at Barnard College in New York City who studies dog cognition, called the wrasse study “amazing.” She added, “I think it … challenges our presumptive notions about what fish can or cannot experience.”
Jordan wants the world to know how smart fish can be. But, he said, “I am the last to say that fish are as smart as chimpanzees. Or that the cleaner wrasse is equivalent to an 18-month-old baby. It’s not.” Rather, he thinks the main point of his paper has more to do with science than fish: “The mirror test is probably not testing for self-awareness,” he said.
[[Hooray! Finally! After decades of mindless hype, a little caution – not to say skepticism.]]

 The question then is what it is doing, and whether we can do better.
What Is Self-Awareness?
Sometimes it’s easy to tell that an animal really doesn’t understand mirrors. The writer Mary Laura Philpott has frequently been awakened in the wee hours of the morning by a loud knocking on her door in Nashville, Tennessee. When she opens the door, she finds only a small turtle. She nicknamed the prankster reptile Frank. Eventually she came to suspect that Frank might be challenging or attacking the strange turtle he sees in the reflective part of her door — night after night after night.
But just because one individual animal fails a mirror test doesn’t mean every member of its species would do the same. It’s a more meaningful positive test than a negative one. And even when animals do recognize themselves in mirrors, researchers are divided about what that implies.
“Recognition of one’s own reflection would seem to require a rather advanced form of intellect,” Gallup wrote in 1970. “These data would seem to qualify as the first experimental demonstration of a self-concept in a subhuman form.”
Either a species shows self-awareness or it doesn’t, as Gallup describes it — and most don’t. “And that’s prompted a lot of people to spend a lot of time trying to devise ways to salvage the intellectual integrity of their favorite laboratory animals,” he told me.
But Reiss and other researchers think self-awareness is more likely to exist on a continuum. In a 2005 study, the Emory University primatologist Frans de Waal and his co-authors showed that capuchin monkeys make more eye contact with a mirror than they do with a strange monkey behind Plexiglas. This could be a kind of intermediate result between self-awareness and its lack: A capuchin doesn’t seem to understand the reflection is itself, but it also doesn’t treat the reflection as a stranger.

Diana Reiss, a cognitive psychologist at Hunter College, has seen evidence of self-recognition in dolphins but she is skeptical about cleaner wrasse: “I think for a claim like this, the evidence has to be much stronger.”
Elizabeth Nolan
Scientists also have mixed feelings about the phrase “self-awareness,” for which they don’t agree on a definition. Reiss thinks the mirror test shows “one aspect of self-awareness,” as opposed to the whole cognitive package a human has. The biologists Marc Bekoff of the University of Colorado, Boulder, and Paul Sherman of Cornell University have suggested a spectrum of “self-cognizance” that ranges from brainless reflexes to a humanlike understanding of the self.
Jordan likes the idea of a spectrum, and thinks cleaner wrasse would fall at the lower end of self-cognizance. He points out that moving your tail before it gets stepped on, or scraping a parasite off your scales, isn’t the same as sitting and pondering your place in the universe. Others in the field have supported his contention that the mirror test doesn’t test for self-awareness, he said. “I think the community wants a revision and a reevaluation of how we understand what animals know,” Jordan said.
One thing on which most scientists in the field do agree is that there’s a link between recognizing yourself in a mirror and being social. The species that perform well on mirror tests all live in groups. In an intriguing 1971 study by Gallup and others, chimpanzees born in captivity and raised in isolation failed the mirror test. The chimps that passed the test had been born in the wild, in social groups. Gallup thought this finding supported the ideas of the philosopher George Herbert Mead of the University of Chicago, who said our sense of self is shaped by our interactions with others. “[T]here could not be an experience of a self simply by itself,” Mead wrote in 1934.
Gallup sees a clear connection between recognizing yourself in a mirror, understanding something about others’ states of mind, and even empathizing. “Once you can become the object of your own attention, and you can begin to think about yourself, you can use your experience to infer comparable experiences in others,” Gallup said. No species evolved looking in mirrors, but some of us can see ourselves reflected in our companions.
The Mirror as a Window
The sociality of Asian elephants helped researchers to design a better mirror test in 2006. Joshua Plotnik, a comparative psychologist now at Hunter College in New York City, worked on the study with de Waal and Reiss. In an earlier test that elephants failed, the animals had been in an enclosure, looking at a small mirror. For the revised test, the researchers used an eight-foot-by-eight-foot mirror, so the elephants could see their whole bodies at once. They also let the elephants approach the mirror so that they could stand on their back legs to look behind it or kneel to peer beneath it.

Asian elephants never seemed to pass the mirror test until experimenters made the mirrors large enough to show their whole body alongside other elephants.
Courtesy of Diana Reiss
They also tested elephants in pairs, which “gave them an opportunity to use their partner as a frame of reference,” Plotnik said. When an elephant saw a friend standing in the mirror next to a stranger, she might be able to deduce that the strange elephant was herself.
This time, one of three elephants passed the test. Plotnik said the researchers have promising results from other elephants that haven’t been published yet.
“You have to really try to take the perspective of the animal that you’re working with,” Plotnik said. For example, elephants like being dirty and might not care about marks on their bodies, unlike grooming animals such as chimpanzees. Gorillas groom, but they hate making direct eye contact with others. This might help explain why they haven’t had the same success in the mirror test as chimps or orangutans.
Plotnik thinks future experiments should take an animal’s particular motivations and perceptions into account. For example, the mirror test is visual, but elephants are more interested in what they smell and hear. “Is it fair if you test an animal that’s not a primarily visual animal and they fail?” Plotnik said. “You could make that argument for dogs.”
Dogs are lousy at recognizing themselves in mirrors. But Horowitz recently designed an “olfactory mirror test” for dogs. She found that dogs spent longer sniffing samples of their own urine when it had an extra scent “mark” added to it.
“It’s challenging for us as visual creatures to imagine ourselves into the sensory worlds of nonvisual animals,” Horowitz said. But we have to do it, she thinks, if we want to understand how their minds work.
Reiss, who calls Horowitz a friend, doesn’t think the olfactory mirror study proves dogs can recognize themselves. But she thinks the experiment is an interesting jumping-off point. “How else can we [design] tests to get glimpses into what animals know about themselves?” she said.
As empathetic as Homo sapiens is, we struggle to place ourselves in the viewpoints of other species. Yet this kind of understanding could help us not just to grasp our own place in the world but to protect the world. For example, Plotnik said, a lack of habitat for Asian elephants is driving conflict between the endangered species and humans. “I think a lot of what’s missing from the debate around how to solve this conflict is the elephant’s perspective,” he said. The kind of insight we get from putting pachyderms in front of mirrors might be a helpful window into their minds.
Several mirrors decorate the walls of Gallup’s office, partially hidden behind the towers of papers. It’s just a coincidence, he told me — the mirrors were there when he moved in. He got up from his chair to show me another coincidence born of pareidolia, our mind’s inclination to look for faces. In the black wood grain of his office door, a student had once pointed out the barely discernible face of a gorilla. Gordon traced it for me: an eye, another eye, two nostrils. He directed me to stand in front of the door and move back and forth until I saw it.
Suddenly the light caught the grain in just the right way and the gorilla’s giant face emerged. It stared back at me directly, as a real gorilla never would, like a glimpse straight into the unknowable mind of an animal. “I do see it!” I said. Gallup laughed delightedly. “Isn’t it amazing?” he asked. Then it was gone.